期刊
ANIMALS
卷 10, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/ani10060930
关键词
EGF; EGFR; fatty acid; Akt; GMEC
资金
- National Natural Science Foundation of China [31672398]
- National Key Research and Development Plan of China [2018YFD0501905]
- Transgenic New Species Breeding Program of China [2018ZX0800805B]
Simple Summary Goat milk contains an abundance of fatty acids which are benefit to human health. Epidermal growth factor (EGF) is a small peptide which could positively regulate the growth, development and differentiation of the mammary gland during lactation. However, little information is available about EGF in regulating lipid metabolism in the mammary gland. This study investigated the effects of EGF on the triglyceride (TG) synthesis, lipogenic genes expression and the downstream signal protein levels in goat mammary epithelial cells (GMECs). Our findings indicated EGF might be beneficial to improve milk fat synthesis of dairy goats. EGF acts as a ligand of the EGF receptor (EGFR) to activate the EGFR-mediated signaling pathways and is involved in the regulation of cell physiology. However, the roles of EGFR mediated signaling pathways in the regulation of lipid metabolism in goat mammary epithelial cells (GMECs) are poorly understood. To evaluate the impact of EGF on GMECs, the triglyceride (TG) content and lipid droplet were detected, using TG assay and immunofluorescence. Further, expression of lipogenic genes, the protein kinase B (Akt), phospholipase C-gamma 1 (PLC-gamma 1) and extracellular signal-regulated kinases (ERK)1/2 signaling pathways were measured by real-time polymerase chain reaction and Western blot, respectively. The results showed that the mRNA expression ofEGFRgene was significantly upregulated in lactating goat mammary gland tissues compared to non-lactation period (p< 0.05). TG contents in EGF-treated GMECs were significantly increased (p< 0.05), and an increase of lipid droplets was also detected. In vitro studies demonstrated that the mRNA levels of lipogenesis-relatedFASN,ACC,SCD1,LXRa,LXRbandSP1genes were positively correlated to the mRNA level of EGFR gene shown by gene overexpression and silencing (p< 0.05). The phosphorylations of Akt, ERK1/2 and PLC-gamma 1 in GMECs were greatly upregulated in the presence of EGF, and specific inhibitors were capable of blocking the phosphorylation of Akt, ERK1/2 and PLC-gamma 1. Compared with EGF-treated GMECs, the mRNA levels ofFASN,ACCandSCD1were significantly decreased in GMECs co-treated with PLC-gamma 1 and Akt inhibitor and EGF (p< 0.05), and TG content was also dropped significantly. These observations implied that EGFR plays an important role in regulating de novo fatty acid synthesis in GMECs, mainly mediated by Akt and PLC-gamma 1 signaling pathways.
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