期刊
INSECTS
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/insects11030171
关键词
Diaphorina citri; transcriptome sequencing; CLas infection
类别
资金
- Natural Science Foundation of China [31960116]
- Ministry of Science and Technology of China [2018YFD0201504]
- Educational Commission of Jiangxi Province of China [GJJ180747]
- Science and Technology Department of Jiangxi Province [20171BCB23074]
- earmarked fund for Jiangxi Agriculture Research System [JXARS-07]
- Key Research and Development Plan of Jiangxi Province [20192BBF60057]
The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important transmission vector of the citrus greening disease Candidatus Liberibacter asiaticus (CLas). The D. citri midgut exhibits an important tissue barrier against CLas infection. However, the molecular mechanism of the midgut response to CLas infection has not been comprehensively elucidated. In this study, we identified 778 differentially expressed genes (DEGs) in the midgut upon CLas infection, by comparative transcriptome analyses, including 499 upregulated DEGs and 279 downregulated DEGs. Functional annotation analysis showed that these DEGs were associated with ubiquitination, the immune response, the ribosome, endocytosis, the cytoskeleton and insecticide resistance. KEGG enrichment analysis revealed that most of the DEGs were primarily involved in endocytosis and the ribosome. A total of fourteen DEG functions were further validated by reverse transcription quantitative PCR (RT-qPCR). This study will contribute to our understanding of the molecular interaction between CLas and D. citri.
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