期刊
MICROBIAL BIOTECHNOLOGY
卷 13, 期 4, 页码 1103-1117出版社
WILEY
DOI: 10.1111/1751-7915.13565
关键词
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资金
- National Natural Science Foundation of China [31802263, 71473019]
- Municipal Key Fund B for Natural Sciences of Beijing Municipal Government [PXM2016_014207_000064]
- National Key Research Plan 'Research on the prevention and control technology of the common diseases of livestock and poultry' [2017YFD0502200]
- Beijing Dairy Industry Innovation Team [BAIC05-2017]
As an inflammation of the endometrium, endometritis can affect fertility and lead to serious economic losses in the dairy industry. Widely found in various tissues and body fluids, exosomes and exosome micro (mi)RNAs have been shown to play an important regulatory role in the immune responses. As one of differentially expressed exosome miRNAs, miR-218 is involved in the pathogenesis of bovine endometritis. The mechanisms of miR-218 in regulating the release of cytokines and chemokines in endometritis, however, are poorly understood. Exosomes were isolated from bovine uterine cavity fluid and verified by transmission electron microscopy. An in vitro lipopolysaccharide-treated cell model for bovine endometritis was then established to evaluate the correlation between exosome-derived miR-218 and the immune responses. We demonstrated that exosomes could be used to deliver miR-218 from endometrial epithelial cells (EECs) into the uterine microenvironment and adjacent recipient cells to modulate local immune responses. miR-218 packaged in the exosomes secreted from EECs acts as an inhibitor by blocking immune factors such as interleukin (IL)-6, IL-1 beta, tumour necrosis factor-alpha, the chemokines macrophage inflammatory genes (MIP)-1 alpha and MIP-1 beta to maintain the immune balance in the uterus. However, uterine inflammation altered the immunoregulatory mechanism of exosome miR-218. MiR-218 is a potential biomarker for the detection of endometritis. Our findings also revealed a new mechanism for the development of endometritis in cows.
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