期刊
ROYAL SOCIETY OPEN SCIENCE
卷 7, 期 4, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsos.191830
关键词
bFGF; sustained release; self-assembling peptide; porous nHA; PA66; bone mesenchymal stem cells
资金
- National Natural Science Foundation of China [81472057]
- Natural Science Foundation of Chongqing Province [KJZH17110]
- Applied Basic Research Programs of Science and Technology Department of Sichuan Province [2018JY0250]
Self-assembled peptide scaffolds based on D-RADA16 are an important matrix for controlled drug release and three-dimensional cell culture. In this work, D-RADA16 peptide hydrogels were coated on artificial bone composed of nano-hydroxyapatite/polyamide 66 (nHA/PA66) to obtain a porous drug-releasing structure for treating bone defects. The developed materials were characterized via transmission electron microscopy and scanning electron microscopy. The proliferation and adhesion of bone mesenchymal stem cells (BMSCs) were examined by confocal laser microscopy and CCK-8 experiments. The osteogenic ability of the porous materials towards bone BMSCs was examined in vitro by staining with Alizarin Red S and alkaline phosphatase, and bioactivity was evaluated in vivo. The results revealed that nHA/PA66/D-RADA16/bFGF reduces the degradation rate of D-RADA16 hydrogels and prolongs sustained release of bFGF, which would promote BMSCs proliferation, adhesion and osteogenesis in vitro and bone repair in vivo. Thus, it deserves more attention and is worthy of further research.
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