期刊
FRONTIERS IN CHEMISTRY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2020.00345
关键词
metal drugs; vanadium; anticancer action; antidiabetic action; proteins; transport in the organism; drug design
资金
- Regione Autonoma della Sardegna [RASSR79857]
- Universita di Sassari (fondo di Ateneo per la ricerca 2019)
- Spanish MINECO [CTQ2017-87889-P]
- Generalitat de Catalunya [2017SGR1323]
Among vanadium compounds with potential medicinal applications, [(VO)-O-IV(acac)(2)] is one of the most promising for its antidiabetic and anticancer activity. In the organism, however, interconversion of the oxidation state to +III and +V and binding to proteins are possible. In this report, the transformation of V-III(acac)(3), (VO)-O-IV(acac)(2), and (VO2)-O-V(acac)2- after the interaction with two model proteins, lysozyme (Lyz) and ubiquitin (Ub), was studied with ESI-MS (ElectroSpray Ionization-Mass Spectroscopy), EPR (Electron Paramagnetic Resonance), and computational (docking) techniques. It was shown that, in the metal concentration range close to that found in the organism (15-250 mu M), V-III(acac)(3) is oxidized to (VO)-O-IV(acac)(+) and (VO)-O-IV(acac)(2), which-in their turn-interact with proteins to give n[(VO)-O-IV(acac)]-Protein and n[(VO)-O-IV(acac)(2)]-Protein adducts. Similarly, the complex in the +IV oxidation state, (VO)-O-IV(acac)(2), dissociates to the mono-chelated species (VO)-O-IV(acac)(+) which binds to Lyz and Ub. Finally, (VO2)-O-V(acac)2- undergoes complete dissociation to give the 'bare' V(V)O2+ ion that forms adducts n[(VO2)-O-V]-Protein with n = 1-3. Docking calculations allowed the prediction of the residues involved in the metal binding. The results suggest that only the (VO)-O-IV complex of acetylacetonate survives in the presence of proteins and that its adducts could be the species responsible of the observed pharmacological activity, suggesting that in these systems (VO2+)-O-IV ion should be used in the design of potential vanadium drugs. If V-III or (VO2)-O-V potential active complexes had to be designed, the features of the organic ligand must be adequately modulated to obtain species with high redox and thermodynamic stability to prevent oxidation and dissociation.
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