Enhanced Biohydrogen Production Influenced by Magnetic Nanoparticles Supplementation Using Enterobacter cloacae

The effects of synthesized magnetic nanoparticles (0-50 mg/L) on biohydrogen production from cotton stalk hydrolysate usingEnterobacter cloacaewere investigated, to find out the enhancement of the bacterial hydrogen production efficiency. The magnetic Fe(3)O(4)NPs with uniform size approximately 50 nm were well synthesized and applied in the fermentative hydrogen production system, accordingly, the addition of optimum concentration of 40 mg/L Fe(3)O(4)NPs resulted in the maximum daily hydrogen production, cumulative hydrogen production, glucose utilization, xylose utilization and hydrogen yield of 462.0 +/- 9.6 mL, 1625.2 +/- 18.0 mL/L, 84.29 +/- 0.34%, 92.36 +/- 0.16%, 0.37 +/- 0.004 mol H-2/mol sugar separately. Moreover, the dynamically fitted parameters of hydrogen production potential (P) and hydrogen production rate (R-m) increased to 1851.92 mL/L and 20.27 mL/(L h) respectively, and the lag phase time shortened to 1.30 h also following the addition of 40 mg/L Fe(3)O(4)NPs as compared with the control treatment (0 mg/L). These results indicated the positive effects of Fe(3)O(4)NPs supplementation on fermentative hydrogen production from cotton stalk hydrolysate byE.cloacae, revealing the feasibility of applying Fe(3)O(4)NPs supplementation for enhancement of such hydrogen production system. [GRAPHICS] .

Automated summary

The addition of 40 mg/L magnetic Fe(3)O(4) nanoparticles significantly enhanced the efficiency of bacterial hydrogen production from cotton stalk hydrolysate, resulting in increased daily and cumulative hydrogen production, glucose and xylose utilization, hydrogen yield, as well as dynamic parameters related to hydrogen production potential and rate.