期刊
STEM CELL REPORTS
卷 14, 期 5, 页码 845-860出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2020.03.014
关键词
-
资金
- DFG [MA7212/2-1, HE3418/8-1, SPP1629]
- Wellcome Trust [104783/Z/14/Z]
- BBSRC [BB/L00402X/1]
- Wellcome Trust [104783/Z/14/Z] Funding Source: Wellcome Trust
- BBSRC [BB/L00402X/1] Funding Source: UKRI
- MRC [MR/K017047/1] Funding Source: UKRI
Adult hippocampal neurogenesis is strongly dependent on thyroid hormone (TH). Whether TH signaling regulates this process in a cell-autonomous or non-autonomous manner remains unknown. To answer this question, we used global and conditional knockouts of the TH transporter monocarboxylate transporter 8 (MCT8), having first used FACS and immunohistochemistry to demonstrate that MCT8 is the only TH transporter expressed on neuroblasts and adult slice cultures to confirm a necessary role for MCT8 in neurogenesis. Both mice with a global deletion or an adult neural stem cell-specific deletion of MCT8 showed decreased expression of the cell-cycle inhibitor P27KIP1, reduced differentiation of neuroblasts, and impaired generation of new granule cell neurons, with global knockout mice also showing enhanced neuroblast proliferation. Together, our results reveal a cell-autonomous role for TH signaling in adult hippocampal neurogenesis alongside non-cell-autonomous effects on cell proliferation earlier in the lineage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据