期刊
NANOMATERIALS
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/nano10051000
关键词
surface-enhanced Raman scattering (SERS); Ag nanoparticles; porous anodized aluminum membrane; Aflatoxin B-1
类别
资金
- National Natural Science Foundation of China [21874029]
- Guangdong Yangfan Program [201635018]
- Guangdong Special Support Program [2017TQ04N706]
- Technology Planning Project of Zhanjiang City [2018A02014, 2016C01002]
- Science and Technology Planning Project of Shenzhen City [JCYJ20170818111719650]
- Industrial Development Special Funds of Dapeng New Area [KY20180202]
- Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) [ZJW-2019-07, ZJW-2019-08]
- Innovation and Development Project about Marine Economy Demonstration of Zhanjiang City [2017C8B1]
- Graduate Education Innovation Planning Project of Guangdong Ocean University [201921]
An Aflatoxin B-1 (AFB(1)) biosensor was fabricated via an Ag nanoparticles assembly on the surface of a porous anodized aluminum (PAA) membrane. First, the Raman reporter 4-Aminothiophenol (4-ATP) and DNA (partially complementary to AFB(1) aptamer) were attached to the surface of Ag nanoparticles (AgNPs) by chemical bonding to form a 4-ATP-AgNPs-DNA complex. Similarly, the surface of a PAA membrane was functionalized with an AFB(1) aptamer. Then, the PAA surface was functionalized with 4-ATP-AgNPs-DNA through base complementary pairing to form AgNPs-PAA sensor with a strong Raman signal. When AFB(1) was added, AgNPs would be detached from the PAA surface because of the specific binding between AFB(1) and the aptamer, resulting in a reduction in Raman signals. The detection limit of the proposed biosensor is 0.009 ng/mL in actual walnut and the linear range is 0.01-10 ng/mL. The sensor has good selectivity and repeatability; it can be applied to the rapid qualitative and quantitative detection of AFB(1).
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