期刊
NANOMATERIALS
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/nano10050992
关键词
nickel nanoparticles; human keratinocytes; cytotoxicity; protein induction; high resolution mass spectrometry
类别
资金
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant [660590]
- Marie Curie Actions (MSCA) [660590] Funding Source: Marie Curie Actions (MSCA)
Although nickel allergy and carcinogenicity are well known, their molecular mechanisms are still uncertain, thus demanding studies at the molecular level. The nickel carcinogenicity is known to be dependent on the chemical form of nickel, since only certain nickel compounds can enter the cell. This study investigates, for the first time, the cytotoxicity, cellular uptake, and molecular targets of nickel nanoparticles (NiNPs) in human skin cells in comparison with other chemical forms of nickel. The dose-response curve that was obtained for NiNPs in the cytotoxicity assays showed a linear behavior typical of genotoxic carcinogens. The exposure of keratinocytes to NiNPs leads to the release of Ni2+ ions and its accumulation in the cytosol. A 6 kDa nickel-binding molecule was found to be synthesized by cells exposed to NiNPs at a dose corresponding to medium mortality. This molecule was identified to be tumor-related p63-regulated gene 1 protein.
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