期刊
FRONTIERS IN PLANT SCIENCE
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2020.00695
关键词
ultrasound; air-coupled ultrasound; resonant spectroscopy; plant leaves; layered plant tissue; leaf's parenchyma layers
资金
- Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) [RTA201500054-C02-01]
- Gobierno de Aragon H09_20R research group
- European Social Fund
- Spanish State Research Agency
- European Regional Development Fund (ERDF/FEDER) [DPI2016-78876-R]
- DOC INIACCAA contract
- INIA
The wide band and air-coupled ultrasonic resonant spectroscopy together with a modified Simulated Annealing metaheuristic algorithm and a 1D layered acoustic-model are used to resolve the structure of plant leaves. In particular, this paper focuses on the extraction of the surface density of the different layers of tissue in leaves having a relatively simple structure. There are three main reasons to select the surface density as the focus of this study: (i) it is a parameter directly extracted by the proposed technique and it requires no further processing, (ii) it is relevant in order to study the dynamic of the water within the different tissues of the leaves and also to study the differential development of the different tissues, and (iii) unlike other parameters provided by this technique (like resonant frequency, impedance, ultrasonic elastic modulus, or ultrasonic damping), this parameter can be easier to understand as it is a direct measure of mass per unit surface. The selection of leaves with a simple structure is justified by the convenience of avoiding an unnecessary complication of the data extraction step. In this work, the technique was applied to determine the surface density of the palisade and spongy parenchyma layers of tissue ofLigustrum lucidum,Vitis vinifera, andViburnum tinusleaves. The first species was used to study the variation of the surface density at full turgor with the thickness of the leaf, while the two other species were used to study the variation of the surface densities with the variation in the leaf relative water content. Consistency of the results with other conventional measurements (like overall surface density, and cross-section optical and cryo-SEM images) is discussed. The results obtained reveal the potential of this technique; moreover, the technique presents the additional advantage that can be appliedin-vivoas it is completely non-invasive, non-destructive, fast, and equipment required is portable.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据