期刊
FRONTIERS IN MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2020.00705
关键词
HpaR; type VI secretion system; Yersinia pseudotuberculosis; oxidative stress; biofilm; aromatic compounds degradation
类别
资金
- National Key R&D Program of China [2018YFA0901200]
- National Natural Science Foundation of China [31670053, 31725003, 31970114, 31671292]
- Open Project Program of the State Key Laboratory of Pathogen and Biosecurity [SKLPBS1825]
- China Postdoctoral Science Foundation [2018M631201]
- Shaanxi Postdoctoral Science Foundation [2018BSHTDZZ20]
HpaR, a MarR family transcriptional regulator, was first identified in Escherichia coli W for its regulation of the hpa-meta operon. Little else is known regarding its functionality. Here, we report that in Yersinia pseudotuberculosis, HpaR negatively regulates the hpa-meta operon similar to in E. coli W. To investigate additional functions of HpaR, RNA sequencing was performed for both the wild-type and the Delta hpaR mutant, which revealed that the type VI secretion system (T6SS) was positively regulated by HpaR. T6SS4 is important for bacteria resisting environmental stress, especially oxidative stress. We demonstrate that HpaR facilitates bacteria resist oxidative stress by upregulating the expression of T6SS4 in Y. pseudotuberculosis. HpaR is also involved in biofilm formation, antibiotic resistance, adhesion to eukaryotic cells, and virulence in mice. These results greatly expand our knowledge of the functionality of HpaR and reveal a new pathway that regulates T6SS4.
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