4.7 Article

Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein Levels

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2020.00158

关键词

hepatitis B virus; DFMO; ODC1; polyamines; HBc

资金

  1. National Key Research and Development Project of China [2018YFE0107500]
  2. National Science Foundation of China [NSFC 81101310]
  3. Natural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0166]
  4. National Sciences Foundation of China [81661148057]
  5. National Science and Technology Major Project Science & Technology Commission of China [2017ZX10202203]
  6. innovative talents promotion plan of Chongqing Municipal Education Commission [CY190405]

向作者/读者索取更多资源

Current treatments of hepatitis B virus (HBV) are limited to Interferon-alpha or the nucleos(t)ide analogs antiviral therapies, and it is crucial to develop and define new antiviral drugs to cure HBV. In this study, we explored the anti-HBV effect of difluoromethylornithine (DFMO), an irreversibly inhibitor of decarboxylase 1(ODC1) on HBV replication. Firstly, we found that polyamines contributed to HBV DNA replication via increasing levels of the HBV core protein (HBc) and capsids. In contrast, depletion of polyamines either by silencing the expression of ODC1 or DFMO treatment, resulted in decreasing viral DNA replication and levels of HBc protein and capsids. Furthermore, we found that DFMO decreased the stability of the HBc protein without affecting mRNA transcription and protein translation. Taken together, our findings demonstrate that DFMO inhibits HBV replication by reducing HBc stability and this may provide a new approach for HBV therapeutics.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据