期刊
ELIFE
卷 9, 期 -, 页码 -出版社
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.53913
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资金
- Ministerio de Economia y Competitividad [RYC-2012-10193, AGL2014-58739-R, AGL2017-84908-R, AGL2015-70159-P, RTI2018-093548-B-I00, AGL2016-71890-REDT]
- H2020 European Research Council [StG 757886-ELONGAN]
- Fundacion Seneca [20887/PI/18]
- Department of Agriculture, Food and the Marine [11/S/104]
- Ministerio de Economia y Competitividad Ramon y Cajal
- European Union Seventh Framework Programme Marie Curie fellowship
- Medical Research Council [MR/M012468/1]
- BBSRC [BB/T006390/1] Funding Source: UKRI
- MRC [MR/M012468/1] Funding Source: UKRI
The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable for sperm-egg membrane fusion: the sperm proteins IZUMO1 and SPACA6, and the egg protein JUNO. Here we demonstrate that another sperm protein, TMEM95, is necessary for sperm-egg interaction. TMEM95 ablation in mice caused complete male-specific infertility. Sperm lacking this protein were morphologically normal exhibited normal motility, and could penetrate the zona pellucida and bind to the oolemma. However, once bound to the oolemma, TMEM95-deficient sperm were unable to fuse with the egg membrane or penetrate into the ooplasm, and fertilization could only be achieved by mechanical injection of one sperm into the ooplasm, thereby bypassing membrane fusion. These data demonstrate that TMEM95 is essential for mammalian fertilization.
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