期刊
POLYMERS
卷 12, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/polym12030615
关键词
structural features; immunomodulatory effect; Passiflora foetida fruits; hetero-galacturonans
资金
- Key Area R&D Program of Guangdong Province [2019B020211002]
- Science and Technology Planning Project of Guangzhou City [201804020077]
- Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2017)
- International Cooperation Program of SCAU [2019SCAUGH03]
- South China Agricultural University Doctoral Students (overseas) Joint Education Programs [2018LHPY003]
Passiflora foetida is a horticultural plant and vital traditional Chinese herbal medicine. In our previous study, the characterization and immuno-enhancing effect of fruits polysaccharide 1 (PFP1), a water-eluted hetero-mannan from wild Passiflora foetida fruits, were investigated. Herein, another three salt-eluted novel polysaccharides, namely PFP2, PFP3, and PFP4, were obtained and structurally characterized. The results showed that PFP2, PFP3, and PFP4 were three structurally similar hetero-galacturonans with different molecular weights of 6.11 x 10(4), 4.37 x 10(4), and 3.48 x 10(5) g/mol, respectively. All three of these hetero-galacturonans are mainly composed of galacturonic acid, galactose, arabinose (75.69%, 80.39%, and 74.30%, respectively), and other monosaccharides including mannose, fucose, glucose, ribose, xylose, and glucuronic acid (24.31%, 19.61, and 25.70%, respectively), although differences in their backbone structure exist. Additionally, immunomodulatory assay indicated that the three hetero-galacturonans possess the ability to promote the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha, and interleukin-6 (IL-6) in RAW264.7 macrophages in a concentration-dependent manner (p < 0.05). Especially, PFP3 displayed a stronger enhancing effect than PFP2 and PFP4 at the minimum effective concentration. Therefore, the results suggested that the obtained three salt-eluted hetero-galacturonans, especially PFP3, could be utilized as immunomodulatory effectivity ingredients in nutritional/pharmaceutical industries.
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