期刊
ADVANCED HEALTHCARE MATERIALS
卷 9, 期 10, 页码 -出版社
WILEY
DOI: 10.1002/adhm.201901860
关键词
dynamic covalent bonds; hydrogels; mechanically imprintable patterns; nanotopography; Schiff base
资金
- European Research Council [ERC-H20202014-ADG-669858]
- Programa Operacional Competitividade e InternacionalizacAo (POCI), in the component FEDER
- national funds (OE) through FundacAo para a Ciencia e a Tecnologia(FCT)/MCTES [PTDC/ BTM-MAT/31498/2017, PTDC/BTM-SAL/30503/2017]
- Portuguese Foundation for Science and Technology/MCTES [UIDB/50011/2020, UIDP/50011/2020]
- Portuguese Foundation for Science and Technology [SFRH/BD/141834/2018]
- Fundação para a Ciência e a Tecnologia [PTDC/BTM-MAT/31498/2017, SFRH/BD/141834/2018, PTDC/BTM-SAL/30503/2017] Funding Source: FCT
Native human tissues are supported by a viscoelastic extracellular matrix (ECM) that can adapt its intricate network to dynamic mechanical stimuli. To recapitulate the unique ECM biofunctionality, hydrogel design is shifting from typical covalent crosslinks toward covalently adaptable networks. To pursue such properties, herein hybrid polysaccharide-polypeptide networks are designed based on dynamic covalent assembly inspired by natural ECM crosslinking processes. This is achieved through the synthesis of an amine-reactive oxidized-laminarin biopolymer that can readily crosslink with gelatin (oxLAM-Gelatin) and simultaneously allow cell encapsulation. Interestingly, the rational design of oxLAM-Gelatin hydrogels with varying aldehyde-to-amine ratios enables a refined control over crosslinking kinetics, viscoelastic properties, and degradability profile. The mechanochemical features of these hydrogels post-crosslinking offer an alternative route for imprinting any intended nano- or microtopography in ECM-mimetic matrices bearing inherent cell-adhesive motifs. Different patterns are easily paved in oxLAM-Gelatin under physiological conditions and complex topographical configurations are retained along time. Human adipose-derived mesenchymal stem cells contacting mechanically sculpted oxLAM-Gelatin hydrogels sense the underlying surface nanotopography and align parallel to the anisotropic nanoridge/nanogroove intercalating array. These findings demonstrate that covalently adaptable features in ECM-mimetic networks can be leveraged to combine surface topography and cell-adhesive motifs as they appear in natural matrices.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据