期刊
ACS SYNTHETIC BIOLOGY
卷 9, 期 6, 页码 1270-1276出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.0c00135
关键词
continuous evolution; continuous culture; orthogonal replication; OrthoRep; eVOLVER
资金
- NIH [1DP2GM119163-01, 1DP2AI131083-01, 1R01EB027793-01]
- NSF [MCB1545158, CCF-1522074]
- DARPA [HR0011-15-2-0031, HR0011-15-C-0091]
- DARPA Biological Robustness in Complex Settings program
We present automated continuous evolution (ACE), a platform for the hands-free directed evolution of biomolecules. ACE pairs OrthoRep, a genetic system for continuous targeted mutagenesis of user-selected genes in vivo, with eVOLVER, a scalable and automated continuous culture device for precise, multiparameter regulation of growth conditions. By implementing real-time feedback-controlled tuning of selection stringency with eVOLVER, genes of interest encoded on OrthoRep autonomously traversed multimutation adaptive pathways to reach desired functions, including drug resistance and improved enzyme activity. The durability, scalability, and speed of biomolecular evolution with ACE should be broadly applicable to protein engineering as well as prospective studies on how selection parameters and schedules shape adaptation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据