4.7 Article

Queuine Micronutrient Deficiency Promotes Warburg Metabolism and Reversal of the Mitochondrial ATP Synthase in Hela Cells

期刊

NUTRIENTS
卷 12, 期 3, 页码 -

出版社

MDPI
DOI: 10.3390/nu12030871

关键词

Queuine; queuosine; micronutrient; microbiome; Warburg metabolism; aerobic glycolysis; RNA modification

资金

  1. Research Frontiers grant from Science Foundation Ireland [07/RFP/BIMF318]
  2. US-Ireland Research and Development - Health Research Board
  3. Science foundation Ireland
  4. National Institute of Health [USIRL-2019-2]
  5. Irish Cancer Society [CRS11FER]
  6. EPITRAN COST Action [CA16120]
  7. Science Foundation Ireland (SFI) [07/RFP/BIMF318] Funding Source: Science Foundation Ireland (SFI)

向作者/读者索取更多资源

Queuine is a eukaryotic micronutrient, derived exclusively from eubacteria. It is incorporated into both cytosolic and mitochondrial transfer RNA to generate a queuosine nucleotide at position 34 of the anticodon loop. The transfer RNA of primary tumors has been shown to be hypomodified with respect to queuosine, with decreased levels correlating with disease progression and poor patient survival. Here, we assess the impact of queuine deficiency on mitochondrial bioenergetics and substrate metabolism in HeLa cells. Queuine depletion is shown to promote a Warburg type metabolism, characterized by increased aerobic glycolysis and glutaminolysis, concomitant with increased ammonia and lactate production and elevated levels of lactate dehydrogenase activity but in the absence of significant changes to proliferation. In intact cells, queuine deficiency caused an increased rate of mitochondrial proton leak and a decreased rate of ATP synthesis, correlating with an observed reduction in cellular ATP levels. Data from permeabilized cells demonstrated that the activity of individual complexes of the mitochondrial electron transport chain were not affected by the micronutrient. Notably, in queuine free cells that had been adapted to grow in galactose medium, the re-introduction of glucose permitted the mitochondrial F1FO-ATP synthase to operate in the reverse direction, acting to hyperpolarize the mitochondrial membrane potential; a commonly observed but poorly understood cancer trait. Together, our data suggest that queuosine hypomodification is a deliberate and advantageous adaptation of cancer cells to facilitate the metabolic switch between oxidative phosphorylation and aerobic glycolysis.

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