期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 22, 期 52, 页码 18742-18746出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201604999
关键词
asymmetrical synthesis; bioorganic chemistry; chemoenzymatic synthesis; glycosylation; N-glycans
资金
- National Institute of General Medical Sciences from the U.S. National Institutes of Health [R01GM090269, P01GM107012]
A divergent chemoenzymaytic approach for the preparation of core-fucosylated and core-unmodified asymmetrical N-glycans from a common advances precursor is described. An undecasaccharide was synthesized by sequential chemical glycosylations of an orthogonally protected core fucosylated hexasaccharide that is common to all mammalian core fucosylated N-glycans. Antennae-selective enzymatic extension of the undecasaccharide using a panel of glycosyl transferases afforded core fucosylated asymmetrical triantennary N-glycan isomers, which are potential biomarkers for breast cancer. A unique aspect of our approach is that a fucosidase (FucA1) has been identified that selectively can cleave a core-fucoside without affecting the fucoside of a sialyl LewisX epitope to give easy access to core-unmodified compounds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据