期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 22, 期 23, 页码 7917-7923出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201600851
关键词
base pair; hybridization; mercury; oligonucleotides; organometallic
资金
- Academy of Finland
- EXPERTS Sustain program
The base-pairing properties of 5-mercuricytosine have been explored at the monomer level by NMR titrations and at the oligonucleotide level by melting temperature measurements. The NMR studies revealed a relatively high affinity for guanine, hypoxanthine, and uridine, that is, bases that are deprotonated upon coordination of Hg-II. Within an oligonucleotide duplex, 5-mercuricytosine formed Hg-II-mediated base pairs with thymine and guanine. In the former case, the duplex formed was as stable as the respective duplex comprising solely Watson-Crick base pairs. Based on detailed thermodynamic analysis of the melting curves, the stabilization by the Hg-II-mediated base pairs may be attributed to a comparatively low entropic penalty of hybridization.
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