期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 22, 期 26, 页码 8955-8965出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201600310
关键词
biological activity; hypoxia; imaging agents; iridium; photochemistry
资金
- 973 Program [2015CB856301]
- National Science Foundation of China [21171177, 21471164, 21525105]
In the present study, four mitochondria-specific and two-photon phosphorescence iridium(III) complexes, Ir1-Ir4, were developed for mitochondria imaging in hypoxic tumor cells. The iridium(III) complex has two anthraquinone groups that are hypoxia-sensitive moieties. The phosphorescence of the iridium(III) complex was quenched by the functions of the intramolecular quinone unit, and it was restored through two-electron bioreduction under hypoxia. When the probes were reduced by reductase to hydroquinone derivative products under hypoxia, a significant enhancement in phosphorescence intensity was observed under one-(lambda = 405 nm) and two-photon (lambda = 720 nm) excitation, with a two-photon absorption cross section of 76153 GM at lambda = 720 nm. More importantly, these probes possessed excellent specificity for mitochondria, which allowed imaging and tracking of the mitochondrial morphological changes in a hypoxic environment over a long period of time. Moreover, the probes can visualize hypoxic mitochondria in 3D multicellular spheroids and living zebrafish through two-photon phosphorescence imaging.
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