期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-020-15126-x
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资金
- Gnotobiology platform of the Institut Pasteur
- France Genomique [ANR-10-INBS-09-09]
- European Research Council (ERC) Advanced Grant BacCellEpi [670823]
- ANR Investissement d'Avenir Programme [10-LABX-62-IBEID]
- ERANET-Infect-ERA PROANTILIS [ANR-13-IFEC-0004-02]
- Fondation Le Roch-Les Mousquetaires
- Danone Research fellowship
- IBISA
- European Research Council (ERC) [670823] Funding Source: European Research Council (ERC)
The intestinal microbiota modulates host physiology and gene expression via mechanisms that are not fully understood. Here we examine whether host epitranscriptomic marks are affected by the gut microbiota. We use methylated RNA-immunoprecipitation and sequencing (MeRIP-seq) to identify N6-methyladenosine (m(6)A) modifications in mRNA of mice carrying conventional, modified, or no microbiota. We find that variations in the gut microbiota correlate with m(6)A modifications in the cecum, and to a lesser extent in the liver, affecting pathways related to metabolism, inflammation and antimicrobial responses. We analyze expression levels of several known writer and eraser enzymes, and find that the methyltransferase Mettl16 is downregulated in absence of a microbiota, and one of its target mRNAs, encoding S-adenosylmethionine synthase Mat2a, is less methylated. We furthermore show that Akkermansia muciniphila and Lactobacillus plantarum affect specific m(6)A modifications in mono-associated mice. Our results highlight epitranscriptomic modifications as an additional level of interaction between commensal bacteria and their host.
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