期刊
WORLD MYCOTOXIN JOURNAL
卷 13, 期 2, 页码 267-275出版社
WAGENINGEN ACADEMIC PUBLISHERS
DOI: 10.3920/WMJ2019.2511
关键词
ochratoxin A; immunochromatographic assay; mycotoxin; immunoassay
资金
- National Key R&D Program of China [2018YFC1602203]
- National Natural Science Funds [NSFC-31671924]
- Jiangxi Province Science Fund for Distinguished Young Scholar [20171BCB23023]
- Natural Science Foundation of Jiangxi, China [20181BAB204019]
- Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-KF-201825]
- Science and Technology Innovation Platform Project of Jiangxi Province [20192BCD40001]
Ochratoxin A (OTA) is a possibly carcinogenic mycotoxin which is one of the most abundant food contaminants. It can be normally monitored and detected by immunochromatographic assays (ICG) which have a good sensitivity and specificity and easy to operate. However, the development of normal ICG is based on traditional OTA-conjugates or chemosynthetic mimotope peptides, which are very high-cost reagents with a detrimental effect on the environment and operators. This study takes advantage of both biosynthetic mimetic OTA-conjugates and a convenient IGG to develop a novel environmental-friendly and low-cost ICG for the rapid detection of OTA. Qualitatively, the visual cut-off level of the developed ICG for OTA was 0.8 ng/ml, which is 10-fold more sensitive than the chemosynthetic mimotope peptide based ICG, while there is no cross-reaction with other mycotoxins. The assay takes only 15 min to acquire results visible to the naked eye. Quantitatively, the half inhibition concentration of the ICG setup with mimetic OTA-conjugates was 0.187 ng/ml, with a linear range of 0.015 to 0.5 ng/ml. These results demonstrate the potential to adapt the method for detecting other toxic mycotoxins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据