期刊
VIRUSES-BASEL
卷 12, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/v12040400
关键词
Arbovirus; Bunyavirales; interferon; neglected diseases; Phenuiviridae; Phlebovirus; reverse genetics; sand fly fever; Toscana virus
类别
资金
- CellNetworks (Heidelberg)
- Deutsche Forschungsgemeinschaft (DFG) [LO-2338/1-1, LO-2338/3-1]
- INRAE starter funds
- IDEX-Impulsion 2020 (University of Lyon)
- FINOVI (Fondation pour l'Universite de Lyon)
- Institut Pasteur, CNRS
- Labex IBEID [ANR-10-IHUB-0002]
- GIS IBiSA (Infrastructures en biologie sante et agronomie) [ANR-13-ISV8-0002-01]
- Heisenberg grant from DFG [BO 4340/1-1, SFB1129, 240245660, SFB 1129]
- Brigitte-Schlieben Lange Program from Baden Wurttemberg, Germany
- DFG [STA 1536/2-1]
- [ANR-18-CE92-0006-02 PHLEBO]
- Agence Nationale de la Recherche (ANR) [ANR-13-ISV8-0002] Funding Source: Agence Nationale de la Recherche (ANR)
The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant lacking expression of the non-structural protein NSs (rTOSV phi NSs). Unlike rTOSV and the wt virus, rTOSV phi NSs was unable to (i) suppress interferon (IFN)-b messenger RNA induction; and (ii) grow efficiently in cells producing IFN-b. Together, our results highlight the importance of NSs for TOSV in evading the IFN response and provide a comprehensive toolbox to investigate the TOSV life cycle in mammalian and insect host cells, including several novel polyclonal antibodies.
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