期刊
XENOBIOTICA
卷 50, 期 10, 页码 1202-1207出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00498254.2020.1761572
关键词
Peimine; drug-drug interaction
Peimine is a major component of Fritillaria ussuriensis, which is a widely used herb in pediatric. It is very common in Chinese traditional medicine to combine with two or more herbs in the clinic. To investigate the effect of peimine on the activity of cytochrome P450 enzymes (CYP450) is necessary for the clinical application of peimine. The effects of peimine on eight human liver CYP isoforms (i.e., 1A2, 3A4, 2A6, 2E1, 2D6, 2C9, 2C19 and 2C8) were investigated in vitro in human liver microsomes (HLMs) with the specific inhibitors as positive control and without peimine or inhibitors as negative control. The enzyme kinetic parameters were calculated. It was found that peimine inhibited the activity of CYP3A4, 2E1, and 2D6 in a concentration-dependent manner with the IC50 values of 13.43, 21.93, and 22.46 mu M, respectively. The inhibition of CYP3A4 was performed in a non-competitive manner with the Ki value of 6.49 mu M, and the inhibition of CYP2E1 and 2D6 was performed in a competitive manner with Ki values of 10.76 and 11.95 mu M. Additionally, peimine inhibited the activity of CYP3A4 in a time-dependent manner with the K-I/K-inact value of 6.17/0.049 min(-1 )mu M-1. Peimine inhibited the activity of CYP3A4, 2E1, and 2D6, which indicated the potential interaction between peimine and drugs metabolized by CYP3A4, 2E1, and 2D6. Further studies are needed to verify the drug-drug interaction and the in vivo effects.
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