The effects of three different microplastics on enzyme activities and microbial communities in soil

Soils always receive microplastics (MPs) from plastic mulching, compost, and sewage irrigation, but the effects of MPs on soil environment remain largely unexplored. The objectives of this study were to investigate the effects of three MPs (membranous polyethylene (PE), fibrous polypropylene (PP), and microsphere PP) on enzyme activities and microbial community structure in one loamy and sandy soil. The concentration of microsphere PP (2 mg/g) was one-tenth of those of the other two MPs (20 mg/g). The results showed that the effects of three MPs on urease, dehydrogenase, and alkaline phosphatase activities followed the order: fibrous PP > membranous PE > microsphere PP, membranous PE > microsphere PP > fibrous PP and fibrous PP > microsphere PP > membranous PE, respectively. Results from high-throughput sequencing of 16S rRNA revealed that the membranous PE and fibrous PP raised the alpha diversities of the soil microbiota, whereas the diversity indexes of microbiota on MPs surfaces were significantly lower than those in the amended soils. MPs significantly altered the microbial community structure, especially for the enrichment of Acidobacteria and Bacteroidetes, the depletion of Deinococcus-Thermus and Chloroflexi. Aeromicrobium, Streptomyces, Mycobacterium, Janibacter, Nocardia, Arthrobacter were prone to inhabit on the MPs surfaces. Practitioner points Three microplastics had different effects on soil enzyme activities. Fibrous PP had a more persistent effect on microbial activity. Membranous PE and fibrous PP raised the alpha diversities of soil microbiota. The effects of membranous PE and fibrous PP on microbial communities were similar. Distinct microbial communities were enriched on the surfaces of microplastics.

Automated summary

This study investigated the effects of three microplastics on soil enzyme activities and microbial community structure, showing that different microplastics had varying effects on enzyme activities, fibrous PP had a more persistent impact on microbial activity, and membranous PE and fibrous PP increased the alpha diversity of soil microbiota.