期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 22, 期 26, 页码 8972-8979出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201600590
关键词
dyes/pigments; energy transfer; fluorescent probes; imaging agents; proteins
资金
- Hungarian Academy of Sciences [LP2013-55/2013]
- Hungarian Scientific Research Fund [NN-110214, NN-116265]
- FEBS Short-Term Fellowship
- [SPP1623]
- [SFB1129]
The synthesis of a set of tetrazine-bearing fluorogenic dyes suitable for intracellular labeling of proteins in live cells is presented. The red excitability and emission properties ensure minimal autofluorescence, while throughbond energy-transfer-based fluorogenicity reduces nonspecific background fluorescence of unreacted dyes. The tetrazine motif efficiently quenches fluorescence of the phenoxazine core, which can be selectively turned on chemically upon bioorthogonal inverse-electron-demand Diels-Alder reaction with proteins modified genetically with strained trans-cyclooctenes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据