Detection of mutant genes with different types of biosensor methods

Mutation is a permanent alteration in the sequence of genomic DNA which its integrity and stability is crucial for all living organism. Recognition of these mutations gives the opportunity to improve the therapeutic of these diseases. The most of detection techniques for point mutations are based on PCR. Despite being highly sensitive, PCR based approaches are suffering from amplification errors caused by mispriming. A biosensor as an analytical device measures biological or chemical reactions, through converting them into electrical signals. Biosensors that detect DNA molecules as the analyte are called genosensors or DNA biosensors. Different types of biosensors and also various piezoelectric sensors such as QCM (quartz crystal microbalance), SAW (Surface Acoustic Wave) with immobilization of sensitivity detectors can be useful devices for detection of mutant genes. In this review, we intend to focus on various biosensor based methods to overcome the disadvantages of conventional methods for detection of point mutations. (C) 2020 Published by Elsevier B.V.