期刊
SENSORS
卷 20, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/s20051501
关键词
Griess assay; colorimetric; nitrite; phenosafranin
资金
- Fundamental Research Funds for the Central University [2018CDYJSY0055]
- Solid-State Fermentation Resource Utilization Key Laboratory of Sichuan Province [2018GTJ010]
- National Natural Science Foundation of China Chongqing Science and Technology Commission [CSTC2018jcyjAX0062]
- Brew Microorganisms Technology and Application of Key Laboratory Project in Sichuan Province [NJ2018-01]
- Chongqing Graduate Tutor Team Construction Project, Analytical and Testing Center of Chongqing University
- Chongqing University
A facile and effective colorimetric-sensing platform based on the diazotization of phenosafranin for the detection of NO2- under acidic conditions using the Griess assay is presented. Diazotization of commercial phenosafranin produces a color change from purplish to blue, which enables colorimetric quantitative detection of NO2-. Optimal detection conditions were obtained at a phenosafranin concentration of 0.25 mM, HCl concentration of 0.4 M, and reaction time of 20 min. Under the optimized detection conditions, an excellent linearity range from 0 to 20 mu M was obtained with a detection limit of 0.22 mu M. Favorable reproducibility and selectivity of the colorimetric sensing platform toward NO2- were also verified. In addition, testing spiked ham sausage, bacon, and sprouts samples demonstrated its excellent practicability. The presented colorimetric sensing platform is a promising candidate for the detection of NO2- in real applications.
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