期刊
PROCESS BIOCHEMISTRY
卷 91, 期 -, 页码 387-397出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2020.01.008
关键词
Eucalyptus-capped-CuO nanoparticle; Breast cancer; ROS; qRT-PCR
资金
- International Scientific Partnership Program ISPP at King Saud University, Saudi Arabia [0031]
This study demonstrates a comprehensive and cost-effective one-step rapid bio-fabrication of Eucalyptus globulus leaf extract (ELE) bio-actives functionalized copper oxide nanoparticles (CuONPs); and analyze its apoptotic and toxic effects in human breast cancer (MCF-7) cells and Aspergillus flavus. The state-of-art characterization data confirmed the thermal-stability of (i) ELE-CuONPs and (ii) surface associated ELE bio-active compounds bearing characteristic functional groups (aromatic-OH, polymeric-OH, C-O, and C=O) over a range of conventional temperatures (30 degrees C-80 degrees C), and microwave (MW) heat (140 degrees C). The MW accelerated ELE-CuONPs (16.9 nm) treated (25 - 100 mu g/ml) MCF-7 cells showed a significant (p < 0.05) reduction in viability due to the dysfunction of mitochondrial membrane potential, intracellular ROS accumulation, and disruption in normal cell cycle progression. Moreover, transcriptional up-regulation of mRNA expression of p53 (8.8 fold), bax (1.42 fold), caspase-3 (2.42 fold), and caspase-9 (3.34 fold) genes in the MCF-7 cells confirmed a mechanistic anti-cancer landscape of synthesized ELE-CuONPs. Furthermore, ELE-CuONPs inhibited sclerotia germination and growth of A. flavus. Conclusively, this study is novel in better demonstrating the formation of ELE-CuONPs that meets the principles of green chemistry. These findings can be used to develop strategies for the management of cancer and fungal infections affecting human health.
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