期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 13, 页码 7516-7523出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1912289117
关键词
CO2 fixation; acetogen; Wood-Ljungdahl pathway; systems biology; glycine synthase-reductase pathway
资金
- Intelligent Synthetic Biology Centre of the Global Frontier Project [2011-0031957]
- C1 Gas Refinery Program through the National Research Foundation of Korea - Ministry of Science and Information Communication and Telecommunication (ICT) (MSIT) [2018M3D3A1A01055733, 2015M3D3A1A01064882]
- National Research Foundation of Korea [2018M3D3A1A01055733] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Among CO2-fixing metabolic pathways in nature, the linear Wood-Ljungdahl pathway (WLP) in phylogenetically diverse acetate-forming acetogens comprises the most energetically efficient pathway, requires the least number of reactions, and converts CO2 to formate and then into acetyl-CoA. Despite two genes encoding glycine synthase being well-conserved in WLP gene clusters, the functional role of glycine synthase under autotrophic growth conditions has remained uncertain. Here, using the reconstructed genomescale metabolic model iSL771 based on the completed genome sequence, transcriptomics, C-13 isotope-based metabolite-tracing experiments, biochemical assays, and heterologous expression of the pathway in another acetogen, we discovered that the WLP and the glycine synthase pathway are functionally interconnected to fix CO2, subsequently converting CO2 into acetyl-CoA, acetyl-phosphate, and serine. Moreover, the functional cooperation of the pathways enhances CO2 consumption and cellular growth rates via bypassing reducing power required reactions for cellular metabolism during autotrophic growth of acetogens.
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