期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 13, 页码 7355-7362出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2000228117
关键词
honey bee; phage; microbiome; Bifidobacterium; metagenomics
资金
- European Research Council ERC-StG MicroBeeOme [714804]
- Human Frontier Science Program Young Investigator grant [RGY0077/2016]
- Swiss National Science Foundation [31003A_179487]
- Japanese metagenomes from Apis mellifera (BioProject) [PRJNA598094]
- Swiss National Science Foundation (SNF) [31003A_179487] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [714804] Funding Source: European Research Council (ERC)
The honey bee gut microbiota influences bee health and has become an important model to study the ecology and evolution of microbiota-host interactions. Yet, little is known about the phage community associated with the bee gut, despite its potential to modulate bacterial diversity or to govern important symbiotic functions. Here we analyzed two metagenomes derived from virus-like particles, analyzed the prevalence of the identified phages across 73 bacterial metagenomes from individual bees, and tested the host range of isolated phages. Our results show that the honey bee gut virome is composed of at least 118 distinct clusters corresponding to both temperate and lytic phages and representing novel genera with a large repertoire of unknown gene functions. We find that the phage community is prevalent in honey bees across space and time and targets the core members of the bee gut microbiota. The large number and high genetic diversity of the viral clusters seems to mirror the high extent of strain-level diversity in the bee gut microbiota. We isolated eight lytic phages that target the core microbiota member Bifidobacterium asteroides, but that exhibited different host ranges at the strain level, resulting in a nested interaction network of coexisting phages and bacterial strains. Collectively, our results show that the honey bee gut virome consists of a complex and diverse phage community that likely plays an important role in regulating strain-level diversity in the bee gut and that holds promise as an experimental model to study bacteria-phage dynamics in natural microbial communities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据