期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 22, 页码 11923-11930出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2002623117
关键词
Janus emulsion; fluorescent dyes; Listeria monocytogenes; biosensing; agglutination
资金
- Vannevar Bush Faculty Fellowship [N000141812878]
- NSF Center for Energy Efficient Electronics Science [ECCS-0939514]
- F32 Ruth L. Kirschstein National Research Service Award
- Funai Overseas Scholarship
Here we report a sensing method for Listeria monocytogenes based on the agglutination of all-liquid Janus emulsions. This two-dye assay enables the rapid detection of trace Listeria in less than 2 h via an emissive signal produced in response to Listeria binding. The biorecognition interface between the Janus emulsions is assembled by attaching antibodies to a functional surfactant polymer with a tetrazine/transcyclooctene click reaction. The strong binding between Listeria and the Listeria antibody located at the hydrocarbon surface of the emulsions results in the tilting of the Janus structure from its equilibrium position to produce emission that would ordinarily be obscured by a blocking dye. This method provides rapid and inexpensive Listeria detection with high sensitivity (<100 CFU/mL in 2 h) that can be paired with many antibody or related recognition elements to create a new class of biosensors.
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