期刊
PLOS ONE
卷 15, 期 3, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0226395
关键词
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资金
- Army Research Office [W911NF-16-1-0169, W911NF-19-1-0298]
- National Science Foundation Graduate Research Fellowship Program [DGE-1842165]
- National Institutes of Health Training Grant via the Biotechnology Training Program at Northwestern University [T32GM008449]
- National Defense Science and Engineering (NDSEG) Fellowship Program - Air Force Research Laboratory
- Office of Naval Research
- Army Research Office
- Ryan Fellowship - Northwestern University
- Air Force Research Laboratory Center of Excellence Grant [FA8650-15-2-5518]
- Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource [NSF ECCS-1542205]
- MRSEC program at the Materials Research Center [NSF DMR-1720139]
- International Institute for Nanotechnology (IIN)
- State of Illinois, through the IIN
- MRI program [NSF DMR1229693]
Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP engineering becomes more common, standardized methods for analyzing changes to MCPs and interpreting results across studies will become increasingly important. In this study, we demonstrate that different imaging techniques yield variations in the apparent size of purified MCPs from Salmonella enterica serovar Typhimurium LT2, likely due to variations in sample preparation methods. We provide guidelines for preparing samples for MCP imaging and outline expected variations in apparent size and morphology between methods. With this report we aim to establish an aid for comparing results across studies.
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