期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 258, 期 -, 页码 1-9出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2016.08.008
关键词
Arthropods; Poison; Neurotoxicity; Neuromuscular blockade; Calcium influx
资金
- CAPES [Edital Toxinologia 063/2010]
- CNPq
- FAPERGS [Edital 001/2013]
- CAPES
Animal venoms have been widely recognized as a major source of biologically active molecules. Bothriurus bonariensis, popularly known as black scorpion, is the arthropod responsible for the highest number of accidents involving scorpion sting in Southern Brazil. Here we reported the first attempt to investigate the neurobiology of B. bonariensis venom (BBV) in the insect and mammalian nervous system. BBV (32 mg/g) induced a slow neuromuscular blockade in the in vivo cockroach nerve-muscle preparations (70 +/- 4%, n = 6, p < 0.001), provoking repetitive twitches and significantly decreasing the frequency of spontaneous leg action potentials (SNCAPs) from 82 +/- 3 min(-1) to 36 +/- 1.3 min(-1) (n = 6, p < 0.05), without affecting the amplitude. When tested in primary cultures of rat hippocampal cells, BBV induced a massive increase of Ca2+ influx (250 +/- 1% peak increase, n = 3, p < 0.0001). The disturbance of calcium homeostasis induced by BBV on the mammalian central nervous system was not accompanied by cellular death and was prevented by the co-treatment of the hippocampal cells with tetrodotoxin, a selective sodium channel blocker. The results suggest that the biological activity of BBV is mostly related to a modulation of sodium channels function. Our biological activity survey suggests that BBV may have a promising insecticidal and therapeutic potential. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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