期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 244, 期 -, 页码 9-15出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2015.11.027
关键词
Aluminum trichloride; Osteoblasts; TGF-beta 1/Smad signaling pathway; Rat; Mineralization
资金
- Science and Technology Program of Heilongjiang Educational Bureau [C201425]
Osteoporosis is a major global public health problem. Aluminum (Al) exposure inhibits osteoblast mineralization and induces osteoporosis. However, the exact mechanism is not fully understood. The transforming growth factor beta 1 (TGF-beta 1)/Smad pathway is a major signaling cascade in regulating osteoblast mineralization. To investigate whether TGF-beta 1/Smad signaling pathway was involved in the Al-induced inhibition of osteoblast mineralization, osteoblasts were cultured and exposed to different concentrations of aluminum trichloride (AlCl3) (containing 0, 0.01, 0.02 and 0.04 mg/mL Al3+) for 24 h. We found that mineralized matrix nodules, mRNA expressions of alkaline phosphatase (ALP), type I collagen (Col I), TGF-beta 1, TGF-beta type I receptor, TGF-beta type II receptor and Smad4, protein expressions of TGF-beta 1 and p-Smad2/3, Smad2/3/4 trimeric complex were all decreased, whereas the mRNA expressions of Smad7 were increased in the AlCl3-treated groups compared with those in control. In conclusion, these results indicated that AlCl3 inhibited osteoblast mineralization via TGF-beta 1/Smad signaling pathway in rat osteoblasts. Our findings could provide novel insights into the mechanisms of action of AlCl3 in osteoporosis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据