期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 248, 期 -, 页码 60-67出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2016.02.008
关键词
Bakuchiol; Myoblast differentiation; MyoD; Myogenic conversion; Muscle regeneration; p38MAPK
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2011-0030074]
- SRC Research Center for Women's Diseases of Sookmyung Women's University
- National Research Foundation of Korea [2011-0030074] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Myoblast differentiation is fundamental to skeletal muscle development and regeneration after injury and defects in this process are implicated in muscle atrophy associated with aging or pathological conditions. MyoD family transcription factors function as mater regulators in induction of muscle-specific genes during myoblast differentiation. We have identified bakuchiol, a prenylated phenolic monoterpene, as an inducer of MyoD-mediated transcription and myogenic differentiation. C2C12 myoblasts treated with bakuchiol exhibit enhanced muscle-specific gene expression and myotube formation. A key promyogenic kinase p38MAPK is activated dramatically by bakuchiol which in turn induced the formation of MyoD/E protein active transcription complexes. Consistently, the recruitment of MyoD and Baf60c to the Myogenin promoter is enhanced in bakuchiol-treated myoblasts. Furthermore, bakuchiol rescues defective p38MAPK activation and myogenic differentiation caused by Cdo-depletion or in RD rhabdomyosarcoma cells. Taken together, these results indicate that bakuchiol enhances myogenic differentiation through p38MAPK and MyoD activation. Thus bakuchiol can be developed into a potential agent to improve muscular regeneration and repair to treat muscular atrophy. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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