期刊
PARASITOLOGY RESEARCH
卷 119, 期 7, 页码 2287-2298出版社
SPRINGER
DOI: 10.1007/s00436-020-06722-3
关键词
Toxoplasma gondii; Differentiation; Bradyzoite; Cyst; Metacaspase 2; Bradyzoite-specific genes
类别
资金
- National Key Research and Development Program of China [2017YFD0501304]
- National Natural Science Foundation of China [31672544, 31730096, 81971964]
Toxoplasma gondii is an important zoonotic protozoan of the phylum Apicomplexa that can infect nearly all warm-blooded animals. The parasite can exist as the interconvertible tachyzoite or bradyzoite forms, leading to acute or latent infection, respectively. No drug has been reported to penetrate the cyst wall and reduce bradyzoite survival and proliferation till now. The transcriptional level of metacaspases 2 (TgMCA2) in T. gondii is significantly upregulated during the formation of bradyzoites in the Pru strain, indicating that it may play an important role in the formation of bradyzoites. To further explore the function of TgMCA2, we constructed a TgMCA2 gene-knockout variant of the Pru strain (Delta mca2). Comparative analysis revealed that the proliferative capacity of Pru Delta mca2 increased, while the invasion and egressing properties were not affected by the knockout. Further data shows that the tachyzoites of Delta mca2 failed to induce differentiation and form bradyzoites in vitro, and the transcriptional levels of some of the bradyzoite-specific genes (such as BAG1, LDH2, and SAG4A) in Delta mca2 were significantly lower compared with that in the Pru strain at the bradyzoite stage. In vivo, no cysts were detected in Delta mca2-infected mice. Further determination of parasite burden in Delta mca2- and Pru-infected mice brain tissue at the genetic level showed that the gene load was significantly lower than that in Pru. In summary, we confirmed that TgMCA2 contributes to the formation of bradyzoites, and could provide an important foundation for the development of attenuated vaccines for the prevention of T. gondii infection.
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