期刊
PARASITOLOGY INTERNATIONAL
卷 75, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2019.102050
关键词
Opisthorchis viverrini; Liver fluke; M60-like metallopeptidase; Mucinase; Glycoprotein
类别
资金
- Faculty of Medicine, Khon Kaen University
- Thailand Research Fund (International Research Network 2018: Liver Fluke Research Network)
- GMS-KKU scholarship
- Thailand Research Funds [TRG5780147]
- Mahidol University
- Mekong Health Science Research Institute, Faculty of Medicine, Khon Kaen University
The carcinogenic liver fluke Opisthorchis viverrini (O. viverrini) is endemic in Thailand and neighboring countries including Laos PDR, Vietnam and Cambodia. Infections with O. viverrini lead to hepatobiliary abnormalities including bile duct cancer-cholangiocarcinoma (CCA). Despite decades of extensive studies, the underlying mechanisms of how this parasite survives in the bile duct and causes disease are still unclear. Therefore, this study aims to identify and characterize the most abundant protein secreted by the parasite. Proteomics and bioinformatics analysis revealed that the most abundant secretory protein is a metallopeptidase, named Ov-M60-like-1. This protein contains an N-terminal carbohydrate-binding domain and a C-terminal M60-like domain with a zinc metallopeptidase HEXXH motif. Further analysis by mass spectrometry revealed that Ov-M60-like-1 is N-glycosylated. Recombinant Ov-M60-like-1 (rOv-M60-like-1) expressed in Escherichia coli (E. coli) was able to digest bovine submaxillary mucin (BSM). The mucinase activity was inhibited by the ion chelating agent EDTA, confirming its metallopeptidase identity. The enzyme was active at temperatures ranging 25-37 degrees C in a broad pH range (pH 2-10). The identification of Ov-M60-like-1 mucinase as the major secretory protein of O. viverrini worms warrants further research into the role of this glycoprotein in the pathology induced by this carcinogenic worm.
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