期刊
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES
卷 30, 期 7, 页码 1216-1226出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.numecd.2020.04.011
关键词
Resveratrol; Human islet; Hypoxia; Apoptosis; Oxidative stress; Function
资金
- Iran National Science Foundation (INSF) [94808805]
- Tehran University of Medical Sciences and Health Services [94-04-87-30223]
- TUMS [9121436008]
Background and aims: Successful islet transplantation as a promising treatment of diabetes type 1 is threatened with the loss of islets during the pre-transplant culture due to hypoxia and oxidative stress-induced apoptosis. Therefore, optimization of culture in order to preserve the islets is a critical point. In this study, we investigated the effect of resveratrol, as a cytoprotective agent, on the cultured human islets. Methods and results: Isolated islets were treated with different concentrations of resveratrol for 24 and 72 h. Islets' viability, apoptosis, apoptosis markers, and insulin and C-peptide secretion, along with the production of reactive oxygen species (ROS), hypoxia inducible factor 1 alpha (HIF-1 alpha), and its target genes in the islets were investigated. Our findings showed that the islets were exposed to hypoxia and oxidative stress after isolation and during culture. This insult induced apoptosis and decreased viability during 72 h. The presence of resveratrol significantly attenuated HIF-1 alpha and ROS production, reduced apoptosis, promoted the VEGF secretion, and increased the insulin and C-peptide secretion. In this regard, resveratrol improved the islet's survival and function in the culture period. Conclusions: Using resveratrol can attenuate the stressful condition for the islets in the pretransplant culture and subsequently ameliorate their viability and functionality that lead to successful outcome after clinical transplantation. (C) 2020 The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据