期刊
NEW PHYTOLOGIST
卷 227, 期 4, 页码 1174-1188出版社
WILEY
DOI: 10.1111/nph.16606
关键词
mu XRF; Bipolaris sorokiniana; Blumeria graminis; cell wall appositions; effector; iron; Prussian blue staining; reactive oxygen species (ROS)
资金
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy [EXC 2048/1, 39068611, DFG ZU 263/2-1]
- Max Planck Society
- Ministry of Education of the Czech Republic
- European Union [CZ.02.1.01/0.0/0.0/15_003/0000336]
- Czech Academy of Sciences [RVO: 60077344]
Like pathogens, beneficial endophytic fungi secrete effector proteins to promote plant colonization, for example, through perturbation of host immunity. The genome of the root endophyte Serendipita indica encodes a novel family of highly similar, small alanine- and histidine-rich proteins, whose functions remain unknown. Members of this protein family carry an N-terminal signal peptide and a conserved C-terminal DELD motif. Here we report on the functional characterization of the plant-responsive DELD family protein Dld1 using a combination of structural, biochemical, biophysical and cytological analyses. The crystal structure of Dld1 shows an unusual, monomeric histidine zipper consisting of two antiparallel coiled-coil helices. Similar to other histidine-rich proteins, Dld1 displays varying affinity to different transition metal ions and undergoes metal ion- and pH-dependent unfolding. Transient expression of mCherry-tagged Dld1 in barley leaf and root tissue suggests that Dld1 localizes to the plant cell wall and accumulates at cell wall appositions during fungal penetration. Moreover, recombinant Dld1 enhances barley root colonization by S. indica, and inhibits H2O2-mediated radical polymerization of 3,3 '-diaminobenzidine. Our data suggest that Dld1 has the potential to enhance micronutrient accessibility for the fungus and to interfere with oxidative stress and reactive oxygen species homeostasis to facilitate host colonization.
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