4.5 Article

A distinct inhibitory mechanism of the V-ATPase by Vibrio VopQ revealed by cryo-EM

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NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 27, 期 6, 页码 589-+

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NATURE PORTFOLIO
DOI: 10.1038/s41594-020-0429-1

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资金

  1. CPRIT Core Facility Support Award [RP170644]
  2. Welch Foundation [I-1561]
  3. Once Upon a Time... Foundation
  4. National Institutes of Health [R01 GM115188]

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The Vibrioparahaemolyticus T3SS effector VopQ targets host-cell V-ATPase, resulting in blockage of autophagic flux and neutralization of acidic compartments. Here, we report the cryo-EM structure of VopQ bound to the V-o subcomplex of the V-ATPase. VopQ inserts into membranes and forms an unconventional pore while binding directly to subunit c of the V-ATPase membrane-embedded subcomplex V-o. We show that VopQ arrests yeast growth in vivo by targeting the immature V-o subcomplex in the endoplasmic reticulum (ER), thus providing insight into the observation that VopQ kills cells in the absence of a functional V-ATPase. VopQ is a bacterial effector that has been discovered to inhibit a host-membrane megadalton complex by coincidentally binding its target, inserting into a membrane and disrupting membrane potential. Collectively, our results reveal a mechanism by which bacterial effectors modulate host cell biology and provide an invaluable tool for future studies on V-ATPase-mediated membrane fusion and autophagy. Cryo-EM visualization of the bacterial pore-forming toxin VopQ bound to its host V-ATPase membrane-domain target reveals how cytotoxic effector proteins promote membrane disruption and inhibit ATPase function.

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