期刊
NANOTECHNOLOGY
卷 31, 期 33, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1361-6528/ab8a8a
关键词
carbon dots; gene therapy; breast cancer; HER2; trastuzumab; resistance
资金
- National Natural Science Foundation of China [61971284, 61671299, 21703267]
- Startup Fund for Youngman Research at SJTU (SFTR at SJTU)
- Foundation Research Project of Jiangsu Province [BK20170423]
- Medical-Engineering Crossover Fund of SJTU [YG2016MS26, YG2019QNA13]
- Instrumental Analysis Center of Shanghai Jiao Tong University
- Center for Advanced Electronic Materials and Devices of Shanghai Jiao Tong University
Dual-targeted therapy in HER2-positive breast cancer cells with the combination of carbon dots/HER3 siRNA and trastuzumab resulted in enhanced antitumor activity, which overcomes the resistance to trastuzumab monotherapy. Herein, we have developed branched polyethylenimine-functionalized carbon dot (BP-CD) nanocarriers, which exhibited efficient green fluorescent protein gene delivery and expression. The positively charged BP-CDs allowed for effective nucleic acid binding and displayed a highly efficient small interfering RNA (siRNA)-mediated delivery targeting of cancer cells. The transfection of BP-CDs and HER3 siRNA complexes down-regulated HER3 protein expression and induced significant cell growth inhibition in BT-474 cells. BP-CDs/HER3 siRNA complexes induced cell death of BT-474 cells through G0/G1 cell cycle arrest and apoptosis. The combined treatment of BP-CDs/HER3 siRNA complexes and trastuzumab caused greater cell growth suppression in BT-474 cells when compared to either agent alone. The findings suggest that this dual-targeted therapy with the combination of BP-CDs/HER3 siRNA and trastuzumab represents a promising approach in breast cancer.
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