期刊
NANO LETTERS
卷 20, 期 4, 页码 2264-2270出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.9b04277
关键词
DNA-PAINT; super-resolution microscopy; Ago-PAINT; single-molecule FRET; Argonaute; DNA origami
类别
资金
- Vidi of The Netherlands Organization for Scientific Research [864.14.002]
- ERC Consolidator Grant of the European Research Council [819299]
- Vrije Programma (SMPS) of the Foundation for Fundamental Research on Matter
- TNO
- European Research Council (ERC) [819299] Funding Source: European Research Council (ERC)
Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of the imager strands. Here we report on a method where imager strands are loaded into a protein, Argonaute (Ago), which allows for faster binding. Ago preorders the DNA imager strand into a helical conformation, allowing for 10 times faster target binding. Using a 2D DNA origami structure, we demonstrate that Ago-assisted DNA-PAINT (Ago-PAINT) can speed up the current DNA-PAINT technique by an order of magnitude, while maintaining the high spatial resolution. We envision this tool to be useful for super-resolution imaging and other techniques that rely on nucleic acid interactions.
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