期刊
MOLECULAR REPRODUCTION AND DEVELOPMENT
卷 87, 期 6, 页码 720-734出版社
WILEY
DOI: 10.1002/mrd.23351
关键词
fsh beta expression; hypothalamic-pituitary-gonadal axis; induced spawning; lh beta expression; maturation-induced steroid levels
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2010/17586-0, 2011/09567-8]
Gonadotropin-releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic-pituitary-gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were characterized at the molecular level and the role of injected GnRHs in vivo was evaluated. The full-length complementary DNA (cDNA) sequences of preproGnRH2 (612 bp) and preproGnRH3 (407 bp) of A. altiparanae were obtained, and the GnRH1 form was not detected. The cDNA sequences of preproGnRH2 and preproGnRH3 were found to be conserved, but a change in the amino acid at position 8 of the GnRH3 decapeptide of A. altiparanae was observed. All the injected GnRHs stimulated lh beta messenger RNA (mRNA) expression but not fsh beta mRNA expression, and only GnRH2 was able to increase maturation-inducing steroid (MIS) levels and possibly stimulate oocyte release. Furthermore, only GnRH2 was able to start the entire reproductive hormonal cascade and induce spawning.
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