期刊
MOLECULAR IMMUNOLOGY
卷 121, 期 -, 页码 144-158出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2020.03.010
关键词
N-linked glycosylation; Fc-gamma receptor; IgG; SPR; Protein-protein interaction; Site-directed mutagenesis
资金
- National Research Council Canada: Human Health Therapeutics Research Center
- Canada Research Chair on Protein-Enhanced Biomaterials
- NSERC
- TransMedTech Institute (NanoBio Technology Platform)
- Canada First Research Excellence Fund
Most of therapeutic monoclonal antibodies belong to the immunoglobulin G1 (IgG1) family; they interact with the Fc gamma receptors (Fc gamma Rs) at the surface of immune cells to trigger effector functions. The IgG1-Fc N-glycans impact the interaction with Fc gamma Rs and are considered a critical quality attribute. Pioneer studies on Fc gamma R N-glycans have unveiled an additional complexity in that the N-glycan linked on the Asn-162 of Fc gamma RIIIa was shown to be directly involved in the strong affinity for afucosylated IgG1. The last few years have thus seen the emergence of many studies investigating the complex influence of Fc gamma RIIIa N-glycans on the interaction with IgG1 through their glycosylation sites or their glycoprofiles. In this context, we performed site-directed mutagenesis along with glycoengineering on Fc gamma Rs (Fc gamma RI, Fc gamma RIIa(H131)/b and Fc gamma RIIIa(V158/F158)) in an effort to elucidate the impact of Fc gamma Rs N-glycans on the interaction with IgG1. Furthermore, we assessed their binding to various trastuzumab glycoforms with an enhanced surface plasmon resonance assay. The Fc gamma RIIIa N-glycans had the highest impact on the interaction with IgG1. More specifically, the N162 glycan positively influenced the affinity (15-fold) for afucosylated IgG1 while the N45 glycan presented a negative impact (2-fold) regardless of the IgG1 glycoforms. Interestingly, only the Fc gamma RIIIa glycoprofile had an impact on the interaction with IgG1 with a 1.5-fold affinity increase when Fc gamma RIIIa displays high-mannose glycans. These results provide invaluable insights into the complex and strong influence of N-glycosylation upon Fc gamma Rs/IgG1 binding and are instrumental to further understand the impact of Fc gamma Rs N-glycosylation in their natural forms.
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