Design and synthesis of novel (Z)-5-((1,3-diphenyl-1H-pyrazol-4-yl)methylene)-3-((1-substituted phenyl-1H-1,2,3-triazol-4-yl)methyl)thiazolidine-2,4-diones: a potential cytotoxic scaffolds and their molecular modeling studies

In an effort to discover potential cytotoxic agents, a series of novel (Z)-5-((1,3-diphenyl-1H-pyrazol-4-yl)methylene)-3-((1-substituted phenyl-1H-1,2,3-triazol-4-yl)methyl)thiazolidine-2,4-dione derivatives (8a-n) were designed and synthesized in various steps with acceptable reaction procedures with quantitative yields and characterized by H-1 NMR, C-13 NMR, IR, HRMS and ESI-MS spectra. These newly synthesized novel derivatives were screened for their in vitro cell viability/cytotoxic studies against human breast cancer cell line (MCF-7) with various concentrations of 0.625 mu M, 1.25 mu M, 2.5 mu M, 5 mu M and 10 mu M, respectively. The biological interpretation assay outcome was demonstrated in terms of cell viability percentage reduction and IC50 values against standard reference drug cisplatin. Based on these results, most of the derivatives exhibited promising cytotoxic activity. Among them, particularly compounds 8j (R-1 = OMe and R-3 = NO2) and 8e (R-3 = CF3) demonstrate remarkable cytotoxic activity with IC50 values 0.426 mu M +/- 0.455 and 0.608 mu M +/- 0.408, which are even better than the standard drug cisplatin 0.636 mu M +/- 0.458 and compounds 8m (R-2 = OMe and R-3 = OMe) and 8c (R-3 = OMe) exhibited closely equivalent IC50 values to the standard drug with IC50 values 0.95 mu M +/- 0.32 and 0.976 mu M +/- 0.313 and rest of the compounds exhibits moderate cytotoxic activity. Moreover, molecular modeling studies and ADME calculations of the novel synthesized derivatives are in adequate consent with the pharmacological screening results.

Automated summary

A series of novel derivatives were designed and synthesized for potential cytotoxic activity against human breast cancer cells, with some showing promising results in comparison to the standard drug cisplatin. Molecular modeling studies and ADME calculations were also in line with the pharmacological screening results.