期刊
MICROCHIMICA ACTA
卷 187, 期 5, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-020-04258-y
关键词
MXene; Ti3C2Tx; Ti3AlC2; beta-Hydroxybutyrate; Biosensor; Amperometric sensor; Ketone bodies; Diabetic ketosis; Diabetic ketoacidosis
资金
- Ministry of Trade, Industry, and Energy [20000580, 10062995]
MXene nanosheets of type Ti3C2Tx were modified with beta-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of beta-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme beta-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of - 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 mu A mM(-1) cm(-2), and a low detection limit (45 mu M). The biosensor was successfully applied to the determination of beta-hydroxybutyrate in (spiked) real serum samples.
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