4.7 Article

Colorimetric determination of polyphenols via a gold nanoseeds-decorated polydopamine film

期刊

MICROCHIMICA ACTA
卷 187, 期 5, 页码 -

出版社

SPRINGER WIEN
DOI: 10.1007/s00604-020-04228-4

关键词

Polydopamine thin film; Seed-mediated growth; Antioxidant capacity; Localized surface plasmon resonance; Colorimetric assay

资金

  1. Ministry of Education, University and Research (MIUR) [AIM1894039-3]
  2. European Social Fund (ESF) [AIM1894039-3]
  3. CSGI

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A polystyrene ELISA plate (EP) modified with a thin film based on gold nanoseeds (AuSDs) assembled onto polydopamine (PDA) is proposed. The nanodecorated film (PDA@AuSD) allows to evaluate the polyphenols antioxidant capacity (AOC) through a colorimetric approach based on a seed-mediated growth strategy. Polyphenols, in the presence of the nanodecorated (PDA@AuSD) surfaces are able to drive an increase in size of the AuSDs according to their AOC; this produces an increase of the localized surface plasmon resonance (LSPR; maximum at lambda 550 nm) that is taken as analytical signal. The PDA@AuSD EP manufacturing shows good intraplates repeatability (RSD <= 6.6%, n = 96 wells) and interplates reproducibility (RSD <= 7.4%, n = 748 wells), resulting stable for 1 year. The AuSDs growth kinetic has been studied using 11 polyphenols belonging to different chemical classes and 4 different food samples. The PDA@AuSD film is able to return quantitative information on the AOC of food polyphenols. Good repeatability (RSD <= 5.7%, n = 12 EP wells) and reproducibility (RSD <= 8.1%, n = 12 EP wells) was achieved, with acceptable linear correlation coefficients (R-2 >= 0.990) and useful limits of detection (LODs <= 2.5 10(-5) mol L-1). The samples analyzed with the PDA@AuSD device have been successfully ordered according to their AOC in agreement with conventional optical methods. The PDA@AuSD plate allows multiple measurements (96 wells per EP) with a one-step strategy, overcoming the limitations related to the use of colloidal nanoparticles; in addition, since absorbance is measured after washing, it is not affected by sample color or turbidity.

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