期刊
MICROCHIMICA ACTA
卷 187, 期 6, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-020-04299-3
关键词
MoS2 nanosheets; graphite microfiber; H2O2 determination; Electrochemical sensor; Peroxidase-like activity
资金
- National Natural Science Foundation of China [51802115]
- Natural Science Foundation of Shandong Province [ZR2018BEM010, ZR2019YQ21]
- Major Program of Shandong Province Natural Science Foundation [ZR2018ZC0843]
- Scientific and Technology Project of University of Jinan [XKY1923]
A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure. The MoS2 nanosheets are synthesized in situ on a graphite microfiber. The chemical composition and surface morphology of the microfiber hybrid structure has been characterized. The microfiber is shown to display peroxidase-mimicking activity. In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode. The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface. The modified microfiber electrode, best operated at a voltage of -0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 mu M concentration range and with a determination limit of 30 nM (at S/N=3). The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
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