期刊
MICROBIOLOGICAL RESEARCH
卷 234, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2020.126426
关键词
L-rhamnose catabolic pathway; RhaR; Inducer; Pectinolytic enzymes; Gene regulation
类别
资金
- NWO [ALWOP.233]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
- Academy of Finland [308284]
In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to Delta lraA, Delta lrlA and Delta lrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in Delta lkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.
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